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p stat2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p stat2
    P Stat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat2/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pmc13039202-279-60-80
    Average 96 stars, based on 186 article reviews
    p stat2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancer.
    Article Snippet: After quantification via BCA assay kit (Thermo Fisher Scientific, Waltham, MA, USA), equal amounts of protein were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were blocked with 5% milk for 2 hours subsequently incubated overnight at 4°C with specific primary antibodies, including: SEMA6D (sc393258) and GAPDH (sc-32233) from Santa Cruz Biotechnology (Dallas, TX, USA); E-cadherin (#3195), Caspase 3 (#3879), Cleaved Caspase 3 (#9585), Cleaved Caspase 8 (#9662), HSP90 (#9715),β-catenin (#8480), Claudin-1 (#13255), N-cadherin (#13116), vimentin (#5741), Plexin A4 (#3816), STAT1 (#14994), p-STAT1(#9167), IRF9 (#76684), STAT2 (#72604), p-STAT2 (#88410), HA tag (#3724) from Cell Signalling Technology (Massachusetts, USA); FLAG (F1804, Sigma-Aldrich); anti-Granzyme B (ab255598, Abcam, Cambridge, UK) and anti-Perforin (ab256453, Abcam, Cambridge, UK). ..

    Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties
    Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: p-JAK1 (Tyr1022/1023, #3331S, 1:1000), JAK1 (#3344 1:1000), p-JAK2 (Tyr1008‚ #8082S, 1:1000), JAK2 (#3230, 1:1000), p-TYK2 (Tyr1054/1055, #68790, 1:1000), TYK2 (#14193, 1:1000), p-STAT1 (Tyr701, #9171, 1:1000), STAT1 (#14994, 1:1000), p-STAT2 (Tyr690‚ #88410, 1:1000), STAT2 (#72604, 1:1000), p-STAT3 (Tyr705, #9145S, 1:500), STAT3 (#9139, 1:1000) and beta-actin (#3700, 1:2000) (all from CST, Frankfurt, Germany). .. HBE ALI lysates were analyzed with primary antibodies against GAPDH (#2118, 1:4000, CST), ISG15 (#sc-166755,1:200, Santa Cruz Biotechnology), Mx1 (#37849, 1:1000, CST), SARS-CoV-2 Nucleocapsid (#40143-R019, 1:10 000, Sino Biological), STAT1 (#9172, 1:1000, CST), phospho-STAT1 Y701 (#9171, 1:1000, CST).

    Article Title: Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancer
    Article Snippet: After quantification via BCA assay kit (Thermo Fisher Scientific, Waltham, MA, USA), equal amounts of protein were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were blocked with 5% milk for 2 h subsequently incubated overnight at 4 °C with specific primary antibodies, including: SEMA6D (sc393258) and GAPDH (sc-32233) from Santa Cruz Biotechnology (Dallas, TX, USA); E-cadherin (#3195), Caspase 3 (#3879), Cleaved Caspase 3 (#9585), Cleaved Caspase 8 (#9662), HSP90 (#9715),β-catenin (#8480), Claudin-1 (#13255), N-cadherin (#13116), vimentin (#5741), Plexin A4 (#3816), STAT1 (#14994), p-STAT1 (#9167), IRF9 (#76684), STAT2 (#72604), p-STAT2 (#88410), HA tag (#3724) from Cell Signaling Technology (Massachusetts, USA); FLAG (F1804, Sigma-Aldrich); anti-Granzyme B (ab255598, Abcam, Cambridge, UK) and anti-Perforin (ab256453, Abcam, Cambridge, UK). ..

    Bioprocessing:

    Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity.
    Article Snippet: .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers. ..

    Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
    Article Snippet: Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers. ..

    Blocking Assay:

    Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties
    Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: p-JAK1 (Tyr1022/1023, #3331S, 1:1000), JAK1 (#3344 1:1000), p-JAK2 (Tyr1008‚ #8082S, 1:1000), JAK2 (#3230, 1:1000), p-TYK2 (Tyr1054/1055, #68790, 1:1000), TYK2 (#14193, 1:1000), p-STAT1 (Tyr701, #9171, 1:1000), STAT1 (#14994, 1:1000), p-STAT2 (Tyr690‚ #88410, 1:1000), STAT2 (#72604, 1:1000), p-STAT3 (Tyr705, #9145S, 1:500), STAT3 (#9139, 1:1000) and beta-actin (#3700, 1:2000) (all from CST, Frankfurt, Germany). .. HBE ALI lysates were analyzed with primary antibodies against GAPDH (#2118, 1:4000, CST), ISG15 (#sc-166755,1:200, Santa Cruz Biotechnology), Mx1 (#37849, 1:1000, CST), SARS-CoV-2 Nucleocapsid (#40143-R019, 1:10 000, Sino Biological), STAT1 (#9172, 1:1000, CST), phospho-STAT1 Y701 (#9171, 1:1000, CST).

    Immunopeptidomics:

    Article Title: USP18 Inhibition Enhances Type I Interferon Signalling and Immune Activation in the Tumour Microenvironment of Triple-Negative Breast Cancer
    Article Snippet: .. Primary antibodies used were STAT1 (14994S, Cell signaling), p-STAT1 (7649S, Cell signaling), STAT2 (MA5-42463, Invitrogen), p-STAT2 (88410S, Cell signaling), USP18 (4813T, Cell signaling), ISG15 (15981-1-AP, ProteinTech), MHC-I (MA5-35712, Invitrogen) and β-actin (A2066-100UL, Sigma Aldrich). .. After washing, the membranes were then incubated with the HRP-linked secondary antibody (7074S, Cell Signaling).



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